Genetically engineered Saccharomyces yeast capable of effective cofermentation of glucose and xylose

Article Abstract:

The most efficient sugar-fermenting microorganisms, Saccharomyces species, could not metabolize the major fermentable sugar xylose which were derived from cellulosic biomass despite the organisms' sugar-fermenting capabilities. The inability of the microorganism to metabolize such sugar to ethanol was due to its inability to use the pentose sugar for aerobic growth. The use of recombinant plasmids that were fused into the microorganism gene had transformed the microorganism into a Saccharomyces xylose-fermenting yeast which can coferment glucose and xylose.

Genetic aspects, Yeast, Saccharomyces, Yeast (Food product), Glucose

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A Bacillus anthracis-based in vitro system supports replication of plasmid pXO2 as well as rolling-circle-replicating plasmids

Article Abstract:

A cell-free system based on Bacillus anthracis that can replicate plasmid DNA in vitro was developed to gain better understanding of the mechanism of replication of pXO2 and other plasmids in Bacillus anthracis. It was seen that replication of pXO2 required directional transcription through plasmid origin of replication and increased transcription through the origin resulted in an increase in plasmid replication.

DNA replication

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Rolling-circle replication of bacterial plasmids

Article Abstract:

Bacterial plasmids studies have made significant contributions to the understanding of the control of DNA replication, recombination, and gene expression. Rolling-circle (RC) plasmids were first discovered in Staphylococcus aureus. Aspects of RC replication and the classification of RC plasmids into groups based on homologies are discussed.

Research, Observations, Bacteria

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