Horizontal spread of mer operons among Gram-positive bacteria in natural environments

Article Abstract:

Mercury resistance (HgR) has bee widely reported and the nucleotide sequences of at least 11 mercury resistance operons (mer) from different bacteria are known. HgR is therefore useful in studying horizontal gene transfer in the environment. The horizontal spread of distinct mer operon variants occurring in diverse Bacillus species, one encoding a mercuric reductase with a single N-terminal domain and the other encoding a reductase with a duplicated N-terminal domain, is reported. Strains with former mer operon types are sensitive to organomercurials, whilst those with the latter types are resistant to organomercurials.

Physiological aspects, Observations, Bacillus (Bacteria), Bacillus, Operons, Mercury, Mercury (Metal), Microbiological research

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Group II intron from Pseudomonas alcaligenes NCIB 9867 (P25X): entrapment in plasmid RP4 and sequence analysis

Article Abstract:

Genetics research using Pseudomonas alcaligenes NCIB 9867 (strain P25X) with plasmid RP4 crossed with Pseudomonas putida NCIB 9869 (strain RA713) without plasmid has isolated group II introns which are known to be directly responsible for genetic mobility. The consensus sequence of GUGYG nucleotides at the 5' end of the chain and RAY at the 3' end of the chain.

Research, Analysis, Nucleotide sequence, Base sequence, Pseudomonas putida, Introns

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