Identification of Mycobacterium paratuberculosis gene expression signals

Article Abstract:

The pYUB76 transcriptional-translational fusion vector was utilized to determine the properties of Mycobacterium paratuberculosis gene expression signals. Analysis of Mycobacterium paratuberculosis chromosomal promoter elements based on transcriptional lacZ fusions in Mycobacterium smegmatis indicated the presence of strong beta-galactosidase activity. Furthermore, the mycobacteriophage promoters have a high degree of G+C content compared to Escherichia coli promoters.

Analysis, Physiological aspects, Genetic aspects, Gene expression, Promoters (Genetics)

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Use of a primate model system to identify Chlamydia trachomatis protein antigens recognized uniquely in the context of infection

Article Abstract:

Research was conducted to examine the use of a primate infection model of chlamydial genital infection with the objective of discovering new Chlamydia trachomatis antigens recognized uniquely in the context of infection. Measurement of serum antibody titres was performed in cynomolgus monkeys challenged urethrally with C. trachomatis serovar L2 elementary bodies (EBs). Two C. trachomatis proteins that are underrepresented in EBs were identified.

Chlamydia trachomatis, Bacterial antigens, O antigens, Kra, Crab eating macaque

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Phage infection, transfection and transformation of Mycobacterium avium complex and Mycobacterium paratuberculosis

Article Abstract:

Initial processes for the genetic manipulation of the pathogens Mycobacterium avium and Mycobacterium paratuberculosis were devised. These bacteria were found to be susceptible to the mycobacteriophage TM4, and were thus amenable to transformation by introducing recombinant DNA. Recombinant plasmids introduced in M. avium and M. paratuberculosis can be used to search for individual cells that carry genes for resistance to kanamycin.

Bacteriophages

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