Identification of a DNA methyltransferase gene carried on a pathogenicity island-like element (VPAI) in Vibrio parahaemolyticus and its prevalence among clinical and environmental isolates

Article Abstract:

A putative virulence-associated DNA methyltransferase (MTase) gene conducted on a novel 22.79-kb pathogenicity island-like element (VPAI) in Vibrio parahaemolyticus is identified. The V. parahaemolyticus MTase gene was shown by PCR to be prevalent (.98%) in pandemic thermostable direct hemolysin gene-positive isolates, which suggests that VPAI might confer unique virulence traits to pandemic strains of V. parahaemolyticus.

Methyltransferases

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Characterization of a Vibrio alginolyticus strain, isolated from Alaskan oysters, carrying a hemolysin gene similar to the thermostable direct hemolysin-related hemolysin gene (trh) of vibrio parahaemolyticus

Article Abstract:

The thermostable direct hemolysin-related hemolysin (trh) like gene is cloned and sequenced and were found 98% identical to the trh2 gene of V. parahaemolyticus. This gene seems to be functional since it was transcriptional active in early stationary-phase growing cells.

Science & research, Research, Bacterial genetics

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Development of a multiplex real-time PCR assay with an internal amplification control for the detection of total and pathogenic Vibrio parahaemolyticus bacteria in oysters

Article Abstract:

The development of a real-time multiplex PCR assay for the simultaneous detection of the thermolabile hemolysin (tlh), thermostable direct hemolysin (tdh), and thermostable-related hemolysin (trh) genes of Vibrio parahaemolyticus is described.

Shellfish, Shellfish Fishing, Oysters, Crassostrea

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