Immunochemical characterization of temperature-regulated production of enterocin L50 (EntL50A and EntL50B), enterocin P, and enterocin Q by Enterococcus faecium L50

Article Abstract:

The generation of specific polyclonal antibodies against chemically synthesized enterocin Q (EntQ) and the C- and N-terminal fragments of enterocin L50A (EntL50A) and enterocin L50B (EntL50B) as well as EntQ, respectively, are described. The analysis has demonstrated the suitability of an immunochemical strategy based on the use of bacteriocin-specific polyclonal antibodies and the results have shown a strong influence of temperature on EntL50, EntP and EntQ production by Enterococcus faecium L50.

Bacterial proteins, Structure, Report

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Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from enterococcus faecium P13 with broad antimicrobial spectrum

Article Abstract:

A novel pediocin-like bacteriocin is isolated, purified and analyzed. The new bacteriocin is called enterocin P and is isolated from a dry fermented Spanish sausage. It is produced by Enterococcus faecium and exhibits a broad antimicrobial spectrum including microorganisms that cause food spoilage. Enterocin P is sec-dependent. The biochemical, molecular and functional properties of this bacteriocin are presented.

Pharmaceutical microbiology

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Complete sequence of the enterocin Q-encoding plasmid pCIZ2 from the multiple bacteriocin producer enterococcus faecium L50 and genetic characterization of enterocin Q production and immunity

Article Abstract:

The locations of the genetic determinants for enterocin L50 (EntL50A and EntL50B), enterocin Q (EntQ) and enterocin P (EntP) were determined in the multiple bacteriocin producer Enterococcus faecium strain L50. It was demonstrated that 2,371-bp fragment from pCIZ2 was sufficient for EntQ production, EntqB was involved in ABC transporter-mediated EntQ secretion while EntqC confered immunity to bacteriocin.

Plasmids, Bacteriocins

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