Intestinal bacterial communities that produce active estrogen-like compounds enterodiol and enterolactone in humans

Article Abstract:

A study with 24 human subjects was undertaken for the characterization of the bacterial communities responsible for secoisolariciresinol (SECO) activation, that is, the communities that can produce the enterolignans enterodiol (ED) and enterlactone (EL). The intestinal bacteria involved in ED production were part of the dominant microbiota as revealed by most-probable-number enumerations.

Ribosomal RNA

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Fluorescence-based bacterial overlay method for simultaneous in situ quantification of surface-attached bacteria

Article Abstract:

A novel method is developed for quantification of surface-attached biotinylated bacteria by in situ detection with fluorescence-labeled avidin-D. The new bacterial overlay method has quantified surface-attached bacteria and can be used for measuring bacterial adherence on material specimens in many applications.

Analysis, Observations, Flow cytometry, In situ hybridization, Scanning electron microscopes

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Degradation of estrogens by Rhodococcus zopfii and Rhodococcus equi isolates from activated sludge in wastewater treatment plants

Article Abstract:

The four strains of Rhodococcus are isolated that specifically degrades estrogens by using enriching culture of activated sludge from wastewater treatment plants. The results suggest that Rhodococcus zopfii and R. equi strains degrade 17beta-estradiol into substances without estrogenic activity.

Microbiological research, Sewage treatment

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