Identification of genes associated with morphology in Aspergillus niger by using suppression subtractive hybridization

Article Abstract:

Suppression subtractive hybridization (SSH) was applied for identifying the genetic elements related with pelleted and filamentous morphologies that are observed in manganese-induced and manganese-suppressed genes in Aspergillus niger and RNA blot analysis was used for studying the responses of the newly isolated genes to the different developmental stages during the fermentation process. The newly isolated genes could be involved in the regulation of Aspergillus niger morphology.

Research and Development in the Physical, Engineering, and Life Sciences, Genetic Engineering

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Genomic comparison of plant pathogenic and nonpathogenic Serratia marcescens strains by suppressive subtractive hybridization

Article Abstract:

The genetic differences responsible for Serratia marcescens pathogenicity in cucurbits are identified by comparing the genomes of a cucurbit yellow vine disease (CYVD) strain, Z01-A, and a rice endophytic strain, R02-A. Primers are designed based on one Z01-A-specific sequence, A79, and used in a multiplex PCR to discriminate between Serratia marcescens strains causing CYVD and those from other ecological niches.

Genetic aspects, Bacteria, Phytopathogenic, Phytopathogenic bacteria, Squashes, Diseases and pests

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Assessment of elasticity and topography of Aspergillus nidulans spores via atomic force microscopy

Article Abstract:

Atomic force microscopy (AFM) was used to investigate both surface topography and micromechanical properties of Aspergillus nidulans spores. The result implies that the rodlet layer is significantly softer than the underlying portion of the cell wall.

Atomic force microscopy

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