Identification of poly(cis-1,4-isoprene) degradation intermediates during growth of moderately thermophilic actinomycetes on rubber and cloning of a functional lcp homologue from Nocardia farcinica strain E1

Article Abstract:

The isolation and characterization of thermophilic bacteria capable of rubber degradation is presented. A gene (lcp) coding for a protein with high homology to Lcp (latex-clearing protein) from Streptomyces sp. strain K30 is identified in Nocardia farcinica E1 and S. lividans TK23 expressing this Lcp homologue is able to cleave synthetic poly(cis-1,4-isoprene), confirming its involvement in initial polymer cleavage.

Synthetic rubber, Synthetic Rubber Manufacturing, Polyisoprene Rubber, Analysis, Bacteria, Thermophilic, Decomposition (Chemistry), Chemical properties, Thermophiles, Nocardia

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Establishment of Tn5096-based transposon mutagenesis in Gordonia polyisoprenivorans

Article Abstract:

The transposons Tn5, Tn10, Tn611 and Tn5096 were characterized regarding transposition in Gordonia polyisoprenivorans strain VH2. One rubber-negative mutant was disrupted in mcr, encoding an alpha-methylacyl-coenzyme A racemase.

Gene mutations, Gene mutation, Transposons, Genetic research, Genetic code

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Biology of the metabolically diverse genus Gordonia

Article Abstract:

Research is conducted to study the Gordonia species of genusEs and a summary is provided regarding its importance. This Gordonia genus plays a pivotal role in industrial and environmental technology.

Green technology, Genu valgus

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