Identification of type A, B, E, and F botulinum neurotoxin genes and of botulinum neurotoxigenic clostridia by denaturing high-performance liquid chromatography

Article Abstract:

Denaturing high-performance liquid chromatography (DHPLC) is a new method for speedy viewing of nucleotide polymorphisms in PCR products, and this method is utilized to check the type A, B, E, and F botulinum neurotoxin genes. Distinct reports were procured for each genus and species, indicating that this method would be a dependable alternative to the progression of speedy detection of food borne pathogens.

Analysis, Microbiology, Genetic polymorphisms, Chromatography

---

Biosensor detection of botulinum toxoid A and staphylococcal enterotoxin B in food

Article Abstract:

Immunoassays were developed for the simultaneous detection of staphylococcal enterotoxin B and botulinum toxoid A in buffer, with limits of detection of 0.1 ng/ml, respectively. The toxins were also spiked and measured in a variety of food samples, including canned tomatoes, sweet corn, green beans, mushrooms, and tuna.

Usage, Biosensors, Enterotoxins

---

Characterization of neutralizing antibodies and identification of neutralizing epitope mimics on the Clostridium botulinum neurotoxin type A

Article Abstract:

Researchers used the phage display method to identify the B-cell epitopes of two monoclonal antibodies against Clostridium botulinum neurotoxin type A. This information could be useful in designing a vaccine against botulism.

Antigenic determinants

Similar abstracts:

• Abstracts: Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers
• Abstracts: Aerobic denitrification of Pseudomonas aeruginosa monitored by online NAD(P)H fluorescence. The gnyRDBHAL cluster is involved in acyclic isoprenoid degradation in Pseudomonas aeruginosa
• Abstracts: Thermal tolerance of Mycobacterium paratuberculosis. Persistence of Mycobacterium parabacterium during manufacture and ripening of Cheddar cheese
• Abstracts: Characterization of Salmonella enterica serovar typhimurium from marine environments in coastal waters of Galicia (Spain)
• Abstracts: A computer-simulated restriction fragment length polymorphism analysis of bacterial small-subunit rRNA genes: efficacy of selected tetrameric restriction enzymes for studies of microbial diversity in nature

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.