Immunocytochemical localization of laccase L1 in wood decayed by Rigidoporus lignosus

Article Abstract:

Immunocytochemical methods were used to determine the location of laccase L1 in Rigidoporus lignosus and in the decaying wood chips of birch tree. Laccase was localized within the cytoplasm and cell wall of R. lignosus, associated with small fibrils. In decaying wood, laccase was localized within the walls of xylem cells, including the middle lamella. Thus, laccase operates in both lignin- and polysaccharide-rich areas. The diffusion of laccase L1 in intact cell walls was hindered. This suggests that the lignocellulose complex has to be previously modified, probably by cellulases, for laccase to diffuse.

Methods, Analysis, Enzymes, Immunocytochemistry

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Identification of Gremmeniella abietina races with random amplified polymorphic DNA markers

Article Abstract:

The use of selected random amplified polymorphic DNA (RAPD) markers differentiates between races of Gremmeniella abietina from the mycelium as well as directly from fruiting bodies. Seven RAPD markers amplified from four oligonucleotides (10-mers) by the polymerase chain reaction were used to distinguish between the North American and European races of Gremmeniella abietina, which is the casual agent of Scleroderris canker of conifers.

Usage, Genetic markers

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A cytochemical study of extracellular sheaths associated with Rigidoporus lignosus during wood decay

Article Abstract:

Extracellular sheaths produced by Rigidoporus lignosus, during host cell colonization, are significant causes for wood degradation, and the Rigidoporus lignosus sheaths may be useful in the recognition of mechanisms in fungal cell-wood surface interactions. Fungal sheaths had a dense or loose fibrillar appearance and were distinct from the fungal cell wall immediately after wood inoculations.

Research, Wood-decaying fungi

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