Improved stress tolerance of GroESL-overproducing Lactococcus lactis and probiotic Lactobacillus paracasei NFBC 338

Article Abstract:

Studies done to identify proteins involved in thermal stress in the probiotic Lactobacillus paracasei NFBC 338 strain using proteomics and to use this knowledge to engineer the probiotic bacteria to overexpress selected proteins involved in the stress response are reported. The results demonstrate the integral role of GroESL in solvent tolerance, and to a lesser extent, thermotolerance of lactic acid bacteria and it is concluded that technologically sensitive cultures, including certain probiotic lactobacilli, could be manipulated to become more robust for survival under harsh conditions, such as food product development and gastrointestinal transit.

All Other Basic Organic Chemical Manufacturing, Industrial Organic Chemicals, Lactic Acid, Probiotics, Lactobacillus plantarum

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Casein-derived antimicrobial peptides generated by Lactobacillus acidophilus DPC6026

Article Abstract:

A study was conducted to investigate the potential of Lactobacillus acidophilus DPC6026 to generate antimicrobial peptides from bovine casein and to assess the activities of these peptides against pathogenic bacteria. The results shows that Lactobacillus acidophilus DPC6026 is suitable for the generation of multiple antimicrobial peptides from casein and that sodium caseinate fermentates produced using Lactobacillus acidophilus DPC6026 might have bioprotective application in milk food products against both gram-positive and gram-negative undesirable bacteria.

Dry, Condensed, and Evaporated Dairy Product Manufacturing, Dry, condensed, evaporated products, Casein, Bacterial proteins, Lactobacillus acidophilus, Chemical properties

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Survival of probiotic lactobacilli in acidic environments is enhanced in the presence of metabolizable sugars

Article Abstract:

Lactobacillus rhamnosus GG is an industrially significant probiotic strain with proven health benefits. The effect of glucose on Lactobacillus rhamnosus GG survival was analyzed to find that the presence of 19.4 mM glucose resulted in up to 6-log (sub10)-enhanced survival following 90 minutes of exposure.

Analysis, Lactobacillus, Bifidobacterium, Bifidobacteria

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