In situ analysis of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes

Article Abstract:

The in situ spatial organization of ammonia-oxidizing and nitrite-oxidizing bacteria in domestic sewage biofilms and autotrophic nitrifying biofilms were studied by employing microsensors and fluorescent in situ hybridization performed with 16S rRNA-targeted oligonucleotide probes. Nitrosomonas bacteria were the dominant ammonia-oxidizing bacteria in both types of biofilms. Nitrospira-like bacteria were the major nitrite-oxidizing bacteria in both biofilms.

Research, Sewage, Sewage microbiology, Bacteria, Nitrifying, Nitrobacteraceae

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In situ activity and spatial organization of anaerobic ammonium-oxidizing (anammox) bacteria in biofilms

Article Abstract:

The autotrophic anaerobic ammonium-oxidizing (anammox) biofilms were investigated for their spatial organization, community composition and in situ activities by molecular biological techniques. Results revealed that Brocadia like anammox bacteria that hybridized with the Amx820 probe dominated with 60-92% of total bacteria found in the upper part of the biofilm where high anammox activity was seen.

Research and Development in the Physical, Engineering, and Life Sciences, Molecular Biology, Analysis, Physiological aspects, Anaerobic bacteria

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