Effects of low-shear modeled microgravity on cell function, gene expression and phenotype in Saccharomyces cerevisiae

Article Abstract:

The behavior of Saccharomyces cerevisiae grown in a high-aspect-ratio vessel, which stimulates the low-shear and microgravity conditions encountered in spaceflight was examined. It was hypothesized that altered gene expression in cells grown in low-shear modeled microgravity might result in changes in the yeast phenotypic and functional characteristics that could be identified and measured by conventional microscopic and fluorescent staining techniques.

Analysis, Gene expression, Cell physiology

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Multiple contributions of peroxisomal metabolic function to fungal pathogenicity in Colletotrichum lagenarium

Article Abstract:

Saccharomyces cerevisiae isocitrate lyase (ICL1) of Colletotricluan lagenarium, which encodes isocitrate lyase involved in the glyoxylate cycle in peroxisornes is isolated and functionally characterized to verify the roles of peroxisome-associated metabolism in fungal pathogenicity. Peroxisomal metabolic pathways play functional roles in appressorial melanization and subsequent host invasion steps that requires the glyoxylate cycle.

Peroxisomes, Cell metabolism

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