Induction of ploidy level increments in an asporogenous industrial strain ofthe yeast Saccharomyces cerevisiae by UV irradiation

Article Abstract:

An increase in ploidy level in an asporogenous strain of Saccharomyces cerevisiae was induced by treatment with ultraviolet radiation (UV). Large-cell clones were selected by microscopy from cell suspensions receiving germicidal UV exposures of more that 39 seconds. Analysis of the cellular DNA contents showed that the large-cell derivatives were either diploid or tetraploids. The results show that UV treatment is a useful procedure for increasing ploidy levels in widely used species of S. cerevisiae.

Ploidy

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Glycerol overproduction by engineered Saccharomyces cerevisiae wine yeast strains leads to substantial changes in by-product formation and to a stimulation of fermentation rate in stationary phase

Article Abstract:

Six commercial wine yeast strains and three nonindustrial strains of Saccharomyces cerevisiae were overexpressed with the GPD1 gene. The gene encodes a glycerol-3-phosphate dehydrogenase and its overexpression resulted in a 1.5 to 2.5 increase in glycerol level but a slight decrease in ethanol formation. The strains have higher amounts of succinate and acetate which vary between the commercial and nonindustrial strains. The concentration of acetoin and 2,3-butanediol were also found to be greater.

Wine and wine making, Yeast fungi, Microbial biotechnology, Yeasts (Fungi), Glycerol, Wine microbiology

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Hydrostatic pressure enhances vital staining with carboxyfluorescein or carboxydichlorofluorescein in Saccharomyces cerevisiae: efficient detection of labeled yeasts by flow cytometry

Article Abstract:

A nonlethal hydrostatic pressure of 30 to 50 MPa causes the extent of intracellular accumulation of carboxyfluorescein or carboxydichlorofluorescein (CDCF) in Saccharomyces cerevisiae to increase five- to 10-fold. This was gleaned from an analysis of individual labeled cells using flow cytometry. Glucose metabolism appears to play a role in the process, since the pressure-induced enhancement of staining with CDCF required D-glucose and its inhibition by 2-deoxy-D-glucose pronounced.

Usage, Flow cytometry, Hydrostatic pressure, Fluorescein

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