Inhibition of homologous recombination by a cohesin-associated clamp complex recruited to the rDNA recombination enhancer

Article Abstract:

The protein interaction network involved in suppressing homologous recombination within the rDNA locus is analyzed using affinity purification combined with highly sensitive mixture mass spectrometry. A model is suggested in which regulator of nucleolar silencing and telophase exit [RENT], Tof2 and Lrs4/Csm1 physically clamp rDNA to the cohesin ring, thereby restricting the movement of rDNA sister chromatids relative to each other to inhibit unequal exchange.

Science & research, Biological Product (except Diagnostic) Manufacturing, Drugs, Recombinant DNA Products, Analytical Laboratory Instrument Manufacturing, Analytical instruments, Ion Mass Spectroscopy, Usage, Recombinant DNA, Mass spectrometry, Gene silencing, Report

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An enzymatic activity in the yeast Sir2 protein that is essential for gene silencing

Article Abstract:

The paper elucidates function of one of the transcriptional silencing proteins Sir2 in yeast. Results demonstrate that Sir2 exhibits an ADP-ribosyltransferase activity that is essential for gene silencing activity and mediates phosphate transfer to itself and to histones in vitro.

United States, Germany, Analysis, Physiological aspects, Gene expression, Genetic transcription, Transcription (Genetics), Yeast, Yeast (Food product), Transferases

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