Isoenzyme multiplicity and characterization of recombinant manganese peroxidases from Ceriporiopsis subvermispora and Phanerochaete chrysosporium

Article Abstract:

A recombinant form of the manganese peroxidase from Phanerochaete chrysosporium can oxidize o-dianisidine and p-anisidine without externally added manganese. The enzyme was expressed in Aspergillus nidulans under the control of the alpha-amylase promoter from Aspergillus oryzae.

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Heterologous expression of laccase cDNA from Ceriporiopsis subvermispora yields copper-activated apoprotein and complex isoform patterns

Article Abstract:

Research has been conducted on Ceriporiopsis subvermispora homokaryon strains. The authors report that genomic clones which encode a putative laccase in these strains have led to allelic variant of lcs-1 gene, and that cDNA clone which corresponds to this gene has been expresed in Aspergillus nidulans and in A. niger, which supports the hypothesis that C. subvermispora has a single laccase gene and that isoforms result from post-translational process.

United States, Chile, Science & research, Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, Physiological aspects, DNA, Fungi, Microbiology, Cloning, Gene expression, Copper, Apoprotein, Apoproteins

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lip-Like genes in Phanerochaete sordida and Ceriporiopsis subvermispora, white rot fungi with no detectable lignin peroxidase activity

Article Abstract:

Two white rot fungi, Phanerochaete sordida and Ceriporiopsis subvermispora, contain lignin peroxidase (LiP)-like sequences but show no LiP activity. The LiP-like genes were identified by PCR amplification of the P. sordida and C. subvermispora genomes. Reverse transcription-PCR shows that the LiP-like gene of P. sordida is transcribed under ligninolytic conditions. However, the transcript is different from the genomic clone. There is no transcription of the C. subvermispora LiP-like gene.

Analysis, Usage, Polymerase chain reaction, Amino acid sequence, Amino acid sequencing

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