Isolation, DNA sequence analysis, and mutagenesis of a proline dehydrogenase gene (putA) from Bradyrhizobium japonicum

Article Abstract:

The proline dehydrogenase gene (putA) from the nitrogen-fixing bacteria Bradyrhizobium japonicum contains an open reading frame that codes for 1,016 amino acids. The putA gene encodes a protein with proline dehydrogenase and pyrroline-5-carboxylate (P5C) dehydrogenase activities like the Escherichia coli and Salmonella typhimurium genes. The B. japonicum putA gene lacks the C-terminal region and is not a part of the operon with proline porter gene unlike the E. coli and S. typhimurium genes. The protein contains the proline dehydrogenase and P5C dehydrogenase catalytic regions.

Observations, Amino acid sequence, Amino acid sequencing, Nitrogen-fixing microorganisms

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Ammonia inhibition of plasmid pRmeGR4a conjugal transfer between Rhizobium meliloti strains

Article Abstract:

Ammonia inhibits plasmid conjugation between Rhizobium meliloti strains. A study of the effect of nutritional factors on two nonsymbiotic plasmids pRmeGR4a and pRmeGR4b of R. meliloti strains indicates that glutamate promotes transconjugation. The conjugation is reduced by a factor of 100 in media with glutamate and ammonium as nitrogen sources, in comparison with media containing only glutamate. The inhibition of conjugal transfer between R. meliloti strains by ammonia is however, unobservable in heterologous plasmids.

Health aspects, Plasmids, Culture media (Biology), Composition, Culture media, Conjugation (Biology), Conjugation (Reproduction), Rhizobium meliloti

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Carbon monoxide dehydrogenase activity in Bradyrhizobium japonicum

Article Abstract:

Research has been conducted on the Bradyrhizobium japonicum strain. The effect of carbon monoxide on the chemolithoautotrophic growth of this strain has been investigated.

Germany, Spain, Statistical Data Included, Bacterial growth, Carbon monoxide, Microbiological research

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