Molecular cloning, sequencing, and expression of a chemoreceptor gene from Leptospirillum ferrooxidans

Article Abstract:

Experiments were conducted to clone and sequence a Leptospirillum ferooxidans 2,262-bp chromosomal DNA fragment displaying a region with high degree of identity with methyl-accepting chemotaxis protein genes. An Escherichia coli DNA-dependent system was used to express the DNA gene in E. coli both in vivo and in vitro. A complete open reading frame observed in the fragment displayed identical characteristics to several chemotactic receptor genes. However, the chemotactic receptor functions were not observed in the fragments expressed in E. coli.

Genetic aspects, Escherichia coli, Cloning

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An immunological strategy to monitor in situ the phosphate starvation state in Thiobacillus ferrooxidans

Article Abstract:

A study was conducted to characterize an immunological technique for analyzing the phosphate limitation condition in individual cells of Thiobacillus ferrooxidans. T. ferrooxidans was grown at 30 degrees C at aph of 1.5 while proteins were determined from Coomassie Blue-stained and heat-dried two-dimensional gels. Experimental results suggested that some substances present in biomining ore-leaching samples may hinder immunological assays.

Analysis, Phosphates, Immunological research, Immunologic research

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Copper ions stimulate polyphosphate degradation and phosphate efflux in Acidithiobacillus ferrooxidans

Article Abstract:

The polyphosphate levels in Acidithiobacillus ferrooxidans, a microorganism with a high level of resistance to heavy metals, are determined. The results support a model for metal detoxification in which heavy metals stimulate polyphosphate hydrolysis and the metal-phosphate complexes formed are transported out of the cell as part of a possibly functional heavy metal tolerance mechanism in A. ferrooxidans.

Science & research, All Other Basic Inorganic Chemical Manufacturing, Phosphorus & Compounds, Microbiological research, Copper ions, Phosphorus compounds

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