Isolation and characterization of the epoxide hydrolase-encoding gene from Xanthophyllomyces dendrorhous

Article Abstract:

The epoxide hydrolase (EH)-encoding gene from Xanthophyllomyces dendrorhous, a basidiomycetous yeast, is discussed based on its isolation and characterization, steps essential for development of a yeast EH-based epoxide biotransformation system. The genomic sequence has a 1,236-bp open reading frame interrupted by eight introns that encode a 411-amino-acid polypeptide with a calculated molecular mass of 46.2 kDa. The amino acid sequence belongs to the alpha/beta hydrolase fold family.

Statistical Data Included, Enzymes, Yeast, Yeast (Food product), Molecular microbiology, Catalysis, Glycols, Glycols (Class of compound)

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High-level production of beta-carotene in Saccharomyces cerevisiae by successive transformation with carotenogenic genes from Xanthophyllomyces dendrorhous

Article Abstract:

Carotenogenic genes from carotenoid-producing yeast Xanthophyllomyces dendrorhous are introduced and expressed in Saccharomyces cerevisiae to determine whether Saccharomyces cerevisiae can serve as a host for efficient carotenoid and especially beta-carotene production. It is observed that a reductase gene (tHMGI) and crtI (phytoene desaturase) are capable of producing high levels of carotenoid and beta-carotene.

Science & research, Food preparations, not elsewhere classified, All Other Miscellaneous Food Manufacturing, Brewers' Yeast, Genetic research, Brewer's yeast, Saccharomyces cerevisiae, Beta carotene

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Metabolic engineering of the carotenoid biosynthetic pathway in the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma)

Article Abstract:

The engineering of the astaxanthin biosynthetic pathway in Xanthophyllomyces dendrorhous by gene activation and over expression of carotenogenic genes. While the former results in the accumulation of intermediates, in case of the latter the carotenoid production levels and carotenoid composition are altered.

Germany, Research and Development in the Physical, Engineering, and Life Sciences, Genetic Engineering, Gene expression, Metabolic regulation, Biosynthesis, Yeast fungi, Yeasts (Fungi)

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