Isolation and characterization of o-xylene oxygenase genes from Rhodococcus opacus TKN14

Article Abstract:

Rhodococcus genes mediating oxygenation in the first step of o-xylene degradation are reported and Rhodococcus opacus TKN14, isolated from soil contaminated with o-xylene is found to utilize o-xylene as the sole carbon source to metabolize it to o-methylbenzoic acid. The results show that the constructed enzyme system is involved in the degradation pathways of a wide range of aromatic hydrocarbons containing benzene substituents and naphthalene substituents by Rhodococcus species as the first key enzyme.

Petrochemical Manufacturing, Xylenes, Genetic aspects, Escherichia coli, Xylene, Chemical properties

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Recovery of spores from thermophilic diary bacilli and effects of their surface characteristics on attachment to different surfaces

Article Abstract:

A method is developed to produce large quantities of endospores from thermophilic bacilli diary isolates and the surface physicochemical characteristics of the endospores are determined. The results are useful in developing strategies to prevent or reduce the number of spores attaching to stainless steel and thereby reducing fouling within a milk powder production plant.

Spores (Bacteria), Bacterial spores, Control, Biophysics, Milk contamination

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Isolation and characterization of thermophilic bacilli degrading cinnamic, 4-coumaric, and ferulic acids

Article Abstract:

The ability of 34 thermophilic Bacillus sp, strains to degrade vanillic acid, an intermediate in the ferulic acid biodegradation pathway, cinnamic, 4-coumaric and others at 60 C is investigated. Results indicate that the organisms use beta-oxidative and non-beta-oxidative mechanisms for degradation of these lignin-related compounds.

Japan, Microbial metabolism, Bacillus (Bacteria), Bacillus, Lignin

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