Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production

Article Abstract:

The isolation and characterization of 2,4-diacetylphloroglucinol (DAPG) produced by Pseudomonas sp. strain F113 was described. An assay based on high-pressure liquid chromatography was developed for quantitative analysis of the DAPG in both culture media and soil. Under the assay conditions, it was shown that DAPG production was optimum at 12 degrees centigrade. The types of carbon sources also affected DAPG production with sucrose, mannitol and fructose providing the highest yields. In addition, a low ratio of culture volume to surface area in a growth container was critical for maximum growth.

Analysis, Antibiotics, Rhizosphere

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Characterization of fluorescent siderophore-mediated iron uptake in Pseudomonas sp. strain M114: evidence for the existence of an additional ferricsiderophore receptor

Article Abstract:

A mutant of Pseudomonas specie strain M114 lacking the outer membrane receptor for ferric pseudobactin M114 was constructed and characterized. This mutant showed lack of an 89- kilodalton protein in the outer membrane and incapability in accumulating ferric pseudobactin M114. Cross-feeding assays showed the presence of an additional ferric pseudobactin receptor for the uptake of iron complexed to pseudobactin MT3A that strain M114 does not produce.

Genetic aspects, Ligands, Ligands (Chemistry), Microbial genetics

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Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens

Article Abstract:

Certain species of the fluorescent Pseudomonas have been found to have biological control of plant root pathogens. The production of siderophores or iron-binding compounds and antibiotic compounds are inhibitory to the plant root pathogens. However, it has been found out that effective biocontrol is dependent on root colonization. Further studies are needed to fully realize the commercial viability of the process.

Usage, Control, Microorganisms, Pseudomonas fluorescens

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