Isolation of beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance

Article Abstract:

A beta-tubulin gene that is isolated, cloned, and sequenced from an ultraviolet irradiated benomyl resistant mutant of Fusarium monoliforme encodes a 446-amino-acid polypeptide similar to other fungal beta-tubulins. Benomyl resistance in the gene is sensitive to cold. Both cold sensitivity and benomyl resistance are caused by mutation at codon 50 in tub2. The mutation leads to amino acid substitution of asparagine for tyrosine at position 50. The expression of the beta-tubulin gene takes place during vegetative growth and conidial germination.

Cloning, Fusarium, Biocompatibility, Benomyl

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Effects of calcium and calmodulin on spore germination and appressorium development in Colletotrichum trifolii

Article Abstract:

The Ca2+/calmodulin signal transduction pathway is necessary for the spore germination and appressorium development of Colletotrichum trifolii on the host plant during the early stages of pathogenesis. Appressorium development on a hard surface and spore germination are necessary steps in the process of fungal development. The disruption of calcium homeostasis by calcium channel inhibitors or ethylene-bis(oxy-ethylenenitrolo)tetraacetic acid blocks appressorium development. Calmodulin suppressors block germination and differentiation.

Calmodulin, Spores (Botany), Spores, Fungi, Pathogenic, Pathogenic fungi, Calcium channel blockers

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Gene expression analysis during conidial germ tube and appressorium development in Colletrichum trifolii

Article Abstract:

Preinfection development in Colletotrichum spp. has three morphologies and is controlled by a complex interplay of environmental signals. Germ tube morphogenesis for Colletotrichum trifolii and the related fungus C. gloeosporioides f. sp. aeschynomene was found to be partially dependent on a balance between self-germination inhibitors and environmental nutrients or cutin. The degree of responsiveness to these environmental signals was very much different between the two fungal species.

Fungi, Gene expression

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