Production of recombinant alpha-galactosidases in Thermus thermophilus

Article Abstract:

Research has been conducted on the Thermus thermophilus. The construction of a Thermus strain suitable for heterologous alpha-galactose production and the practical value of this strain are described.

Galactose

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Isolation of toxigenic Nocardiopsis strains from indoor environments and description of two new Nocardiopsis species, N. exhalans sp. nov. and N. umidischolae sp. nov

Article Abstract:

Research has been conducted on the Nocardiopsis strains isolated from water-damaged indoor environments. Results indicate that these isolates have produced toxins detected via the boar spermatozoa used as test cells.

United States, Finland, Australia, Toxins, Spermatozoa, Sperm

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Cloning of the gene encoding a novel thermostable alpha-galactosidase from Thermus brockianus ITI360

Article Abstract:

The cloning and sequencing of an alpha-galactosidase gene from the thermophilic bacterium Thermus brockianus ITI360 as well as the purification and characterization of the recombinant enzyme were conducted. The objective was to investigate a thermostable alpha-galactosidase from Thermus bacteria, to isolate the encoding gene and to characterize the recombinant protein. The enzyme was observed to have a high affinity for oligomeric substrates. Results indicate that no alpha-galactosidase activity could be detected in the crude extracts of the mutant strain.

Research, Genetic aspects, Bacteria, Thermophilic, Cloning, Recombinant proteins, Thermophiles

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