Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogeneity

Article Abstract:

Length heterogeneity analysis by polymerase chain reaction (LH-PCR) was employed to determine the composition of bacterioplankton populations from a water sample from off the Oregon coast. Length variability in small-subunit rDNAs of bacterial strains were examined to trace the phylogenetic origins of the domains amplified by LH-PCR. Findings showed that the relative gene frequencies are highly reproducible when PCR production concentrations are restricted to relatively low values. However, at high concentrations, the kinetic bias causes large distortions in gene frequencies.

Analysis, Usage, Water, Aquatic microbiology, Polymerase chain reaction, Plankton

---

A novel delta-subdivision proteobacterial lineage from the lower ocean surface layer

Article Abstract:

A small-subunit ribosomal RNA (16S rRNA) gene lineage (SAR324) related to the delta subdivision of the class Proteobacteria (DP) was found in a 16S rRNA gene clone library prepared from a water sample obtained from 250 m in the western Sargasso Sea. Phylogenetic analysis revealed that SAR324 was most closely related to the DP, although it had no specific affinity to any DP 16S rRNA genes in databases.

Ribosomal RNA

---

Representative freshwater bacterioplankton isolated from Crater Lake, Oregon

Article Abstract:

Isolation and characterization of bacterioplankton representatives of typical, abundant phylotypes in Crater Lake is described. The ultraoligotrophic status of Crater Lake is likely to favor the survival of organisms able to switch between heterotrophy and phototrophy as light availability and organic carbon levels fluctuate.

Science & research, Oregon, Planktonic bacteria, Freshwater zooplankton

Similar abstracts:

• Abstracts: Stratification of activity and bacterial community structure in biofilms grown on membranes transferring oxygen
• Abstracts: Genetic variation of southern hemisphere fur seals (Arctocephalus spp.): investigation of population structure and species identity
• Abstracts: Phosphorylation and functions of the RNA polymerase II CTD. Gene-specific requirement for P-TEFb activity and RNA polymerase II phosphorylation within the p53 transcriptional program
• Abstracts: Crystal structure of the cys2 activator-binding domain of protein kinase C-delta in complex with phorbol ester
• Abstracts: An examination of cetacean brain structure with a novel hypothesis correlating thermogenesis to the evolution of a big brain

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.