Lysine-mannitol-glycerol agar, a medium for the isolation of Salmonella spp., including S. typhi and atypical strains

Article Abstract:

The medium, lysine-mannitol-glycerol agar (LMG) has the combined features of xylose-lysine-deoxycholate agar and mannitol-lysine-crystal violet-brilliant agar, yet glycerol is added for the differentiation of Salmonella and Citrobacter spp. The medium allows the detection of strains with atypical fermentation patterns, such as the lactose or sucrose-positive salmonella and also detects Salmonella typhi after enrichment. The incubation time of LMG is short and the different properties allow selection of suspect isolates and the frequency of isolation of Salmonella spp. is heightened through lower selective pressure.

Research, Salmonella typhosa, Salmonella typhi, Agar

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Construction of Bordetella pertussis strains that overproduce genetically inactivated pertussis toxin

Article Abstract:

The integration of copies of the tox(super Lys9Glu129) operon into the chromosome of Bordetella pertussis at the tox or fha locus by unmarked allelic exchange was described. This method generated strains that secreted high levels of nontoxic analogues of pertussus toxins (PT) proportional to the number of intergrated operons. These strains can be used in industrial-scale production of whooping cough vaccine containing a genetically detoxified PT analogs.

Prevention, Genetic aspects, Bordetella pertussis, Whooping-cough, Whooping cough, Pertussis toxin

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Characterization of the polyurethanolytic activity of two Alicycliphilus sp. strains able to degrade polyurethane and N-methylpyrrolidone

Article Abstract:

The ability of two Alicycliphilus sp. Strains to use N-methylpyrrolidone as a source of carbon and nitrogen and to devaluate polyurethane with an esterase activity is presented.

Plastics Material and Resin Manufacturing, Plastics materials and resins, Urethane Resins, Urethane Resins ex Elastomer & Foam, Physiological aspects, Growth, Gram-positive bacteria, Fourier transform infrared spectroscopy, Polyurethanes, Company growth, Structure, Nucleotide sequencing

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