Manganese peroxidase mRNA and enzyme activity levels during bioremediation of polycyclic aromatic hydrocarbon-contaminated soil with Phanerochaete chrysosporium

Article Abstract:

The fungus Phanerochaete chrysosporium shows a high level of transcription of the three manganese peroxidase (MnP) genes during growth on soil containing polycyclic aromatic hydrocarbons (PAHs). After a brief lag period, the high mnp transcription is followed by a high level of MnP enzyme activity. The expression of mnp and MnP correlate with the degradation of fluorene and chrysene. The rate of biodegradation decreases after ten days of fungal growth, despite the presence of MnP and mnp transcripts. This indicate that MnP-dependent pathways can degrade high ionization potential PAHs.

Research, Physiological aspects, Biodegradation, Fungi, Polycyclic aromatic hydrocarbons

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Structure, organization, and transcription of a cellobiohydrolase gene cluster from Phanerochaete chrysosporium

Article Abstract:

A cluster of three structurally related cellobiohydrolase (cbh) genes from Phanerochaete chrysosporium was sequenced and characterized. Whereas P. chrysosporium cbh1 and cbh2 shared significant homology with Trichoderma reesei cbh1, P. chrysosporium cbh1 lacked the cellulose-binding domain common to other microbial cellulases. The transcript levels of the three genes, especially P. chrysosporium cbh1-3, varied considerably under different culture conditions.

Enzymes, Wood-decaying fungi

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Expression of lip genes during growth in soil and oxidation of anthracene by Phanerochaete chrysosporium

Article Abstract:

Expression of the genes for lignin peroxidase (lip) in anthracene-transforming soil cultures of Phanerochaete chrysosporium was investigated at the mRNA level by competitive reverse transcription-polymerase chain reaction. The results revealed a significant relationship between transcript abundance and protein synthesis. Except for one gene, all 10 members of lip gene family exhibited identical patterns of transcript abundance.

Fungi, Pathogenic, Pathogenic fungi

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