Whole-cell hybridization of Frankia strains with fluorescence- or digoxigenin-labeled, 16S rRNA-targeted oligonucleotide probes

Article Abstract:

Frankia strains in pure cultures and in nodules is detected and identified by the use of whole-cell hybridization with non-radioactively labeled oligonucleotide probes. After the removal of the polysaccharide capsule surrounding the vesicles, identification of Frankia vesicles in nodule homogenates is possible. Specific hybridization is facilitated by incubation with H2O2 (15% in water for 1 h at room temperature) before lyzozyme and detergent treatments.

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Development of an oligonucleotide probe targeting 16S rRNA and its application for detection and quantitation of the ruminal bacterium Synergistes jonesii in a mixed-population chemostat

Article Abstract:

The development of radiolabelled and fluorescent-dye-conjugated oligonucleotide probes which targeted 16S rRNA for the enumeration of the ruminal bacterium Synergistes jonesii 78-1 in mixed culture was described. Both probes were highly specific for the target organism complementary sequence. The results indicate that rRNA-targeted probes could be used for tracking bacteria in the rumen ecosystem.

Research, Ribosomal RNA, Bacteriology, Rumen, Rumen microbiology

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