Manure enhances plasmid mobilization and survival of Pseudomonas putida introduced into field soil

Article Abstract:

Polymerase chain reaction (PCR) was utilized to determine the occurrence of Inc groups in manured and nonmanured soil to determine the possibility of an entry of broad-host-range plasmids via manure into the soil. PCR-based detection of IncN-, IncW-, IncQ- and IncP-specific sequences in soil indicated the role of manure in enhancing the prevalence of the plasmids. Furthermore, the presence of IncW plasmids in manured soil indicated the presence of plasmid entry into soil via manure.

Environmental aspects, Polymerase chain reaction, Manures, Genetic vectors

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A relative of the broad-host-range plasmid RSF1010 detected in Erwinia amylovora

Article Abstract:

The first report of the presence of a sulfonamide and streptomycin resistance plasmid in a plant pathogenic bacteria is presented. The plasmid pEa8.7 found in Erwinia amylovora was analyzed by polymerase chain reaction. Sequencing, hybridization and restriction fragment length polymorphism studies reveal a strong homology between this plasmid and RSF1010, a broad host range plasmid found in many bacteria.

Genetic aspects, Bacteria

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