Mechanism of force generation of a viral DNA packaging motor

Article Abstract:

Optical tweezers are used to study, at the single-molecule level, the mechanism of force generation of Bacillus subtilis phage phi 29 DNA packaging motor. The kinetic parameters of the packaging motor and their dependence on external load is determined to reveal that DNA translocation does not occur during the adenosine triphosphate (ATP) binding but is likely triggered by phosphate release.

United States, Genetic aspects, Bacillus subtilis, Translocation (Genetics), Translocations (Genetics)

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A structure-based model for the synthesis and hydrolysis of ATP by F(sub 1)-ATPase

Article Abstract:

A detailed structure based kinetic model for the mechanism of action of F(sub 1)-ATPase was presented. The results show that further tests were required for the model that represented the first consistent description of how that bifunctional enzyme was able to perform both ATP synthesis and ATP hydrolysis.

Hydrolysis, ATP synthesis

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