Mercury methylation and demethylation in anoxic lake sediments and by strictly anaerobic bacteria

Article Abstract:

Mercury methylation and demethylation experiments in sediments of some affected lakes in Pine Barrens, NJ, collected from May to Nov 1996 were conducted. The study was aimed at determining the factors that influence the balance of the opposing methylation and demethylation processes and which are responsible for the overall environmental methylmercury concentrations. High mercury methylation and high demethylation potentials were noted in the same sediment, which correlate positively with organic matter and dissolved sulfate concentrations in the sediment and with mercury levels in fish.

Analysis, Environmental aspects, Marine sediments, Mercury, Mercury (Metal), Methylation, Pine Barrens (New Jersey)

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Characterization of a defined 2,3,5,6-tetrachlorobiphenyl-ortho-dechlorinating microbial community by comparative sequence analysis of genes coding for 16S rRNA

Article Abstract:

A defined microbial community was characterized using comparative sequence analysis of genes coding for 16S rRNA for the identification of potential polychlorinated biphenyl (PCB)-dechlorinating anaerobes that ortho dechlorinate 2,3,5,6-tetrachlorobiphenyl. Protocols were developed for chromosomal DNA extraction from sediment. Results provide evidence for the applicability of the selective enrichment techniques with molecular monitoring approach for the monitoring and selection of highly defined PCB-dechlorinating microbial consortia.

Polychlorinated biphenyls, Ribosomal RNA, Genetic code

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Complete mineralization of benzene by aquifer microorganisms under strictly anaerobic conditions

Article Abstract:

Subsurface microbes were found to completely mineralize benzene to carbon dioxide under completely anaerobic conditons. Degradation began after at least 30 days. The rate of degradation increased up to an initial benzeneconcentration of 140 millimoles, but higher concentrations had longer lag times and lower degradation rates which are attributed to substrate toxicity. Although the terminal electron acceptor for this process has not been identified, sulfate is a likely candidate.

Petroleum

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