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Modelling the growth limits (growth/no growth interface) of Escherichia coli as a function of temperature, pH, lactic acid concentration, and water activity

Article Abstract:

A probability model for defining the growth/no growth interface was applied to a nonpathogenic strain of Escherichia coli for pH, water activity, temperature and lactic acid concentration conditions that are suboptimal for growth. In broth where the lactic acid concentration was beyond 11 mM or, with two exceptions, at a pH of 3.9 or less with no lactic acid present, Escherichia coli exhibited an inability to grow. The model proved to be useful in describing the growth/no growth interface.

Author: Ratkowsky, D.A., Ross, T., Presser, K.A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998

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Modelling the growth rate of Escherichia coli as a function of pH and lactic acid concentration

Article Abstract:

The growth rate responses of Escherichia coli M23 to suboptimal pH and lactic acid concentration were investigated. Without lactic acid, E. coli grew at pH 4.0 but not a pH 3.7 and did not grow in the presence of >/= 8.32 mM undissociated lactic acid. Growth rate was linearly related to hydrogen ion concentration in the absence of lactic acid. Growth rate was also linearly related to undissociated lactic acid concentration in the range 0 to 100 mM lactic acid.

Author: Ratkowsky, D.A., Ross, T., Presser, K.A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
Lactic acid

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Growth limits of Listeria monocytogenes as a function of temperature, pH, NaCl, and lactic acid

Article Abstract:

Researchers have developed a model that can predict the growth of Listeria monocytogenes given the temperature, water activity, pH, and lactic acid levels in the medium. This can be used to ensure the safety of foods that become contaminated with Listeria.

Author: Tienungoon, S., Ratkowsky, D.A., McMeekin, T.A., Ross, T.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
Safety and security measures, Food, Growth, Listeria monocytogenes, Food safety

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Subjects list: Research, Escherichia coli, Bacterial growth
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