Molecular analysis of the recA gene and SOS box of the purple non-sulfur bacterium Rhodopseudomonas palustris no. 7

Article Abstract:

A study was conducted to analyze the recA gene of Rhodopseudomonas palustris and the construction of the recA mutant. The regulation of the recA gene in the strain was examined. The nucleotide sequence was shown to support 1089 bp encoding a polypeptide of amino acids. The promoter region oft eh gene was delimited using a sequential deletion of the fusion plasmid. Findings showed that R palustris no 7 recA gene is inducible by DNA damage.

DNA damage, Nucleotides, Bacteria, Photosynthetic, Photosynthetic bacteria

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A single V317A or V317M substitution in enzyme II of a newly identified beta-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose

Article Abstract:

Research describes a beta-glucoside phosphotransferase system in the actinobacterium Corynebacterium glutamicum R. The system is composed of three genes encoding phosphotransferase system, beta-glucosidase, and the transcription regulator. Data indicate that these enzymes facilitate utilization of beta-glucosides and by a single amino acid substitution in the phosphotransferase system specificity towards cellobiose is achieved.

Japan, Physiological aspects, Microbial metabolism, Genetic regulation, Carbohydrate metabolism, Enzyme regulation

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High-throughput transposon mutagenesis of Corynebacterium glutamicum and construction of a single-gene disruptant mutant library

Article Abstract:

A simple and high-throughput transposon-mediated mutagenesis system employing two different types of transposons in combination with direct genomic DNA amplification and thermal asymmetric interlaced PCR (TAIL-PCR) is developed. This method is useful in furthering genetic understanding of cellular life and facilitating the functional analysis of microorganisms.

Science & research, Polymerase chain reaction, Transposons, Structure, Chemical properties, Corynebacteria

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