Molecular assays for detecting Aphanomyces invadans in ulcerative mycotic fish lesions

Article Abstract:

Sensitive PCR and fluorescent peptide nuclei acid in situ hybridization (FISH) assays were developed to detect and identify Aphanomyces invadans and to use the assays to screen populations of infected fish. Neither the FISH assay nor the PCR assay cross-reacted with other closely related oomycetes providing strong evidence that the pathogenic oomycete is the primary etiological agent in ulcerative mycosis, an ulcerative skin disease caused by a fungus-like agent of wild and cultured fish.

Commercial Fishing, Fishing, Edible Fish, Physiological aspects, Fish as food, Fish (Food product), In situ hybridization

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Aquatic plants stimulate the growth of a biofilm formation by Mycobacterium ulcerants in axenic culture and harbor these bacteria in the environment

Article Abstract:

Mycobacterium ulcerans is a pathogen that causes Buruli ulcer a necrotic skin disease prevalent throughout Western Africa and studies indicate that aquatic insects could be transmitting this pathogen. Studies conducted show that crude extracts from the green algae added to BACTEC 7H12B culture medium, reduced to half.

Growth, Aquatic plants, Company growth

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Aquatic snails, passive hosts of Mycobacterium ulcerans

Article Abstract:

A few aquatic snails that may transitorily harbor Mycobacterium ulcerans without offering favorable conditions for its growth and replication are reported. Results indicate that no mycobacterial growth was detected in aquatic snails and so they may be a passive host of M. ulcerans and other environmental mycobacteria.

Genetic aspects, Aquatic animals, DNA replication

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