Reduced proteolysis of secreted gelatin and Yps1-mediated alpha-factor leader processing in the Pichia pastoris kex2 disruptant

Article Abstract:

A study aims to isolate and disrupt the Pichia pastoris genes encoding kex2 and Yps1 and to evaluate the utility of these genes disruptants for the production of intact Col1a1-2 gelatin. The different substrate specificities of both proteases and their mutual redundancy for propeptide processing indicate that Pichia pastoris kex2 and yps1 single-gene disruptants can be used for the alpha-factor leader-directed secretion of heterologous proteins otherwise degraded at basic residues.

All Other Miscellaneous Chemical Product and Preparation Manufacturing, Chemical preparations, not elsewhere classified, Gelatin, Analysis, Proteolysis

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The cyclic AMP receptor protein modulates colonial morphology in Vibrio cholerae

Article Abstract:

The deletion of cyclic AMP receptor protein (CMP) and adenylate cyclase (cya) has converted a rugose quorum-sensing regulator HapR mutant to a smooth one. The results have helped in devising a model for the regulatory input of CRP on expolysaccharide biosynthesis.

Genetic aspects, Cyclic adenylic acid, Cyclic adenosine monophosphate

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Viral preprotoxin signal sequence allows efficient secretion of green fluorescent protein by Candida glabrata, Pichia pastoris, Saccharomyces cerevisiae and Schizosaccharomyces pombe

Article Abstract:

Some yeast species are developed into an important host for processing or secretion or recombitant protein that can be used therapeutically and commercially. Foreign protein secretions of four distantly related yeasts were studied and results are discussed.

In-Vitro Diagnostic Substance Manufacturing, Diagnostic substances, Viral Reagents, Case studies, Causes of

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