Molecular characterization of a toluene-degrading methanogenic consortium

Article Abstract:

A toluene-degrading methanogenic consortium enriched from creosote-contaminated aquifer material has been characterized with a molecular approach. Six eubacterial sequences and two archaeal sequences were found to be the most abundant ones based on RFLP analysis Relative abundance of the putatitve species was estimated by use of fluorescent in situ hybridization (FISH). It is likely, based on results, that a eubacterial spcies related to the genus Desultfotomaculum initiates the processing of toluene. Other species detected are likely related to genera Methanosaeta and Methanospirillum.

Canada, Statistical Data Included, Physiological aspects, Environmental aspects, Petroleum, Biodegradation, Toluene, Molecular microbiology

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The methanogenic archaeon Methanosarcina thermophila TM-1 possesses a high-affinity glycine betaine transporter involved in osmotic adaptation

Article Abstract:

The uptake of betaine by Methanosarcina thermophila TM-1 was studied. Results showed that M. thermophila gathered betaine in concentrations up to 140 times those of a concentration gradient within 10 min. of exposure to solute when cells were grown in mineral salts media with 0.1 to 0.8 M NaCl. The betaine uptake system was composed of a single, high-affinity transporter with an apparent K(sub s) of 10 microM and an apparent maximum transport velocity of 1.15 nmol/min/mg of protein.

Glycine

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