Toxic effects on bacterial metabolism of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride

Article Abstract:

The monotetrazolium redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), used to stain actively respiring bacteria, adversely affects bacterial growth and respiration. Samples treated with CTC have lower heterotrophic bacterial plate counts, bacterial production, and 14C glucose incorporation as compared to untreated control samples. Luminescence in a Microtox bioassay also demonstrates the toxicity of CTC. This implies that the CTC technique may provide wrong results while determining bacterial metabolic processes.

Health aspects, Stains and staining (Microscopy), Stains (Microscopy)

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Methanogenic transformation of methylfurfural compounds to furfural

Article Abstract:

The methanogenic bacterium Methanococcus strain B uses the 5- and 2-methylfurfural compounds as growth substrate to convert them stoichiometrically into furfural. The metabolic transformation uses methyl group as a carbon source and carries out demethylation. This metabolic conversion finds application in anaerobic treatment of methylfurfural containing waste waters. Methylfurfural acts as an electron donor for methanogenesis.

Sewage, Organic compounds, Sewage treatment

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Evidence for isotopic exchange during metabolism of stable-isotope-labeled formate in a methanogenic sediment

Article Abstract:

The stable-isotope-labeled and nonlabeled formate in freshwater methanogenic sediment is removed at the rate of 240 + or - 15 and 400 + or - 40 micro moles per hour, respectively. The removal of deuterium and 13C-labeled formate occurs with a simultaneous formation of the nonlabeled formate. The isotopic exchange due to the activity of dehydrogenase is responsible for the formation of nonlabeled formate.

Isotopes, Dehydrogenases, Oxidoreductases, Labels, Lake sediments

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