Molecular detection of streptomycin-producing streptomycetes in Brazilian soils

Article Abstract:

Actinomycetes were isolated from soybean rhizosphere soil gathered from two field sites in Brazil. All isolates belonged to the Streptomyces species and were screened from streptomycin production and the presence of two genes, strA and strB1, which are needed in streptomycin biosynthesis in Streptomyces griseus. There were two major clusters, with the majority of resistant strains from cultivated sites in cluster 1. Only representatives from this cluster had strA. Streptomycetes with strA and strb1 were phenotypically diverse, and only half could be assigned to known species.

Streptomyces, Streptomycin

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Detection of infectious tobamoviruses in forest soils

Article Abstract:

Research was conducted to analyze elution and bait plant techniques of detecting infectious tobamoviruses in forest soils in New York State. It is hypothesized that infectious tobamoviruses can be detected using the two methods in forest soils collected from stands where virus-infected trees exist. Results support the hypothesis that infectious tobamoviruses are present in soils of forest stands in which infected trees occur.

Methods, Soil microbiology, Forest soils, Plant viruses

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Detection of infectious astroviruses in water

Article Abstract:

A new method is proposed for detecting wild-type astroviruses in water from an area where a gastroenteritis epidemic was reported. The method is based on the infection of CaCo-2 cells and a molecular hybridization technique with a specific cDNA probe. It is very useful for differentiating infectious and non-infectious particles in the environment.

Water, Aquatic microbiology, Marine bacteria, Gastroenteritis, Sanitary microbiology

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