Molecular identification and localization of filamentous symbiotic bacteria associated with the hydrothermal vent annelid Alvinella pompejana

Article Abstract:

The specificity and sensitivity of polymerase chain reaction (PCR) primers for the 13B and 5A proteobacterial phylotypes was utilized to determine if the phylotypes are ubiquitous features of Alvinella pompejana. Diagnostic PCR of Alvinella pompejana samples from the East Pacific Rise indicate that phylotypes 13B and 5A are both filamentous bacteria which are prominent features of the hair-like structures on the dorsal integumen of the annelid. Furthermore, the filamentous bacteria associated with Alvinella pompejana were identified as members of the epsilon Proteobacteria.

Analysis, Bacteria, Identification and classification, Ribosomal RNA, Tube worms, Tubeworms, Microbial populations

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Abundance of reverse tricarboxylic acid cycle genes in free-living microorganisms at deep-sea hydrothermal vents

Article Abstract:

Studies were done to demonstrate the abundance of the reverse tricarboxylic acid cycle genes in diverse vent samples using PCR with degenerate primers for three key genes in the reverse tricarboxylic acid cycle and form I and form II of ribulose 1,5-bisphosphate carboxylase/oxygenase. The results also suggest that the reverse tricarboxylic acid cycle has a major autotrophic pathway in the environments and contribute significantly to global autotrophic process.

Science & research, Research, Biological diversity, Biodiversity, Microbiological research, Carboxylic acids

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