Monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16S rRNA gene-based analyses

Article Abstract:

A dual culture-dependent and direct molecular approach combining single-strand conformation polymorphism (SSCP) fingerprinting and sequencing of 16S rRNA genes were used to investigate the diversity and dynamics of bacterial populations in Saint-Nectaire, a raw-milk, semihard cheese. Fingerprinting DNA isolated from milk by SSCP analysis yielded complex patterns, whereas analyzing DNA isolated from cheese resulted in patterns composed of a single peak which corresponded to that of lactic acid bacteria.

Dairy Cattle and Milk Production, In-Vitro Diagnostic Substance Manufacturing, Dairy farms, Raw Milk, Bacterial Dairy Cultures, Analysis, Nucleic acids, Nucleic acid isolation, Dairy bacterial cultures

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Improved assessment of denitrifying, N2-fixing, and total-community bacteria by terminal restriction fragment length polymorphism analysis using multiple restriction enzymes

Article Abstract:

A computer program, termed TReFID, was developed to allow identification of any of 17,327 GenBank sequences by means of polygons generated from the specific terminal restriction fragments (tRFs) of each bacterium. The results revealed that noncultured denitrifying and dinitrogen-fixing bacteria might play a more dominant role in soils.

Nucleotide sequence, Base sequence, Polygons

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Fecal bacterial diversity in a wild gorilla

Article Abstract:

The bacterial diversity in fecal samples of a wild gorilla is described by using a 16S rRNA gene clone library and terminal-restriction fragment length polymorphism (T-RFLP). The results have indicated that fecal populations did not change temporarily, as examined by T-RFLP.

Genetic aspects, Spirochetes, Gorillas

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