Glycoprotein hypersecretion alters the cell wall in Trichoderma reesei strains expressing the Saccharomyces cerevisiae dolichylphosphate mannose synthase gene

Article Abstract:

The results of a biochemical characterization of Trichoderma reesei strains obtained by overexpression of the yeast dolichylphosphate mannose synthase gene are presented and the role of cell wall morphology and increased glycosylation in protein secretion is discussed. It is revealed that the carbohydrate composition of the fungal cell wall is altered with an increased amount N-acetylglucosamine, suggesting an increase in chitin content.

Poland, Analysis, Physiological aspects, Observations, Fluorescence microscopy, Gene expression, Glycosylation, Yeast fungi, Yeasts (Fungi)

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Saccharomyces cerevisiae-based molecular tool kit for manipulation of genes from gram-negative bacteria

Article Abstract:

A tool kit of vectors was designed for manipulating and expressing genes from a wide range of gram-negative species by Saccharomyces cerevisiae in vivo recombination. The analysis suggested that Saccharomyces cerevisiae could use its native recombination proteins for combining several amplicons in a single transformation step with high efficiency.

New Hampshire, Gram-negative bacteria, Bacterial transformation

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