Multicopy integration of heterologous genes, using the lactococcal group II intron targeted to bacterial insertion sequences

Article Abstract:

The use of lactococcal group II intron, LI.ltrB is described, to achieve multicopy delivery of heterologous genes into the genome of Lactococcus lactis IL1403-UCD without the need for selectable markers. The highest level of green fluorescent protein (GFP) expression was observed from a specific six-copy variant that produced GFP at a level analogous to that obtained with a multicopy plasmid.

Genetic aspects, Bacterial genetics, Introns, Lactococcus

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Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies

Article Abstract:

The effects of temperature, salt concentration and formamide concentration on the emission characteristics of commonly used fluorescent labels were evaluated on DNA microchips. The temperature change affected the results obtained during melting curves analysis in DNA microarray studies.

Ribosomal RNA, DNA microarrays

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Noninvasive pigment identification in single cells from living phototrophic biofilms by confocal imaging spectrofluorometry

Article Abstract:

Analysis of fluorescent pigments of a single cell is made with a new imaging technique based on confocal scanning laser microscopy. This technique is used along with a spectrofluorometric detector.

Scanning electron microscopes

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