New multiple-deletion method for the Corynebacterium glutamicum genome, using a mutant lox sequence

Article Abstract:

A new markerless-deletion method based on a Cre/mutant lox system combining a right-element (RE) mutant lox site with a left-element (LE) mutant lax site is used for large-scale genome rearrangements in Corynebacterium glutamicum. The Cre/loxP system using a pair of mutant lox sites provides a new, efficient genome rearrangement method for C. glutamicum and it also facilitates the understanding of genome functions of microorganisms.

United States, Genomes, Microbial genetics

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Isolation and characterization of a native composite transposon, Tn14751, carrying 17.4 kilobases of Corynebacterium glutamicum chromosomal DNA

Article Abstract:

The intrinsic characteristics of a native 20.3-kb composite transposon, Tn14751, which carries approximately 17.4kb of Corynebacterium glutamicum chromosomal DNA is reported. It is concluded that a derivative of this element carrying a kanamycin resistance cassette, minicomposite Tn14751, transposed into the genome of C. glutamicum at an efficiency of 1.8 x 10(super 2) transformants per ug of DNA.

Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, DNA, Kanamycin

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Large-scale engineering of the Corynebacterium glutamicum genome

Article Abstract:

The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. A precise genome excision method that successfully deleted 11 distinct genomic regions identified by comparative analysis of Corynebacterium glutamicum genomes was developed to construct an improved Corynebacterium glutamicum genome.

Analysis, Microbial genetic engineering

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