Nisin dissipates the proton motive force of the obligate anaerobe Clostridium sporogenes PA 3679

Article Abstract:

The effects of the antimicrobial protein, nisin, on the proton motive force in Clostridium sporogenes were investigated. Nisin was found to dissipate the cell's transmembrane potential and pH gradient. The pH gradient was ameliorated by cation release from the treated cells. The transmembrane potential was also decreased by nisin treatment. A decrease in the transmembrane potential without a compensatory pH change produced complete depletion of the proton motive force. Furthermore, a rapid decrease in the amount of intracellular adenosine triphosphate was noted. Cell viability and colony-forming ability were likewise impaired.

Antibiotics

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Nisin resistance in Listeria monocytogenes ATCC 700302 is a complex phenotype

Article Abstract:

Research was performed to evaluate the roles of both the cytoplasmic membrane and the cell wall in a single nisin-resistant strain of Listeria monocytogenes and also to find out whether nisin resistance leads to intrinsic resistance to other antimicrobials. Evidence suggests that the nisin-resistant strain was sensitive to certain cell wall-acting compounds even when grown in the absence of nisin. Furthermore, nisin resistance in L. monocytogenes ATCC 700302 conferred cross-resistance to both class IIa bacteriocin pediocin PA-1 and class IV leuconocin S.

Bacteria, Listeria monocytogenes, Bacterial motility

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Characterization of the bactericidal effects of sodium nitroprusside and other pentacyanonitrosyl complexes on the food spoilage bacterium Clostridium sporogenes

Article Abstract:

Research was conducted to examine the inhibitory effect of sodium nitroprusside (SNP) on Clostridium sporogenes. Objective was to determine the mode of bactericidal action of SNP at the cellular level at the concentrations used for the growth inhibition studies. Cell growth in anaerobic culture was measured using standard methods of optical density and viable cell counts. Cell morphology was studied using light and transmission electron microscopy. Results suggest the cell wall surface to be the primary point of attack of the nitrosyl complex.

Sodium nitroferricyanide, Nitroprusside

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