Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair

Article Abstract:

Proteins that are tagged ubiquitin-conjugating enzyme variants (UEV) lack the active-site residues found in the E2 proteins they resemble. A UEV protein that is MMS2-encoded is examined to determine its role in error-free postreplicative DNA repair in Saccharomycetes cerevisiae. It is shown that Mms2p forms a heterodimeric complex with the UBC-13-encoded E2 and is needed for the Ubc 13p-dependent assembly of polyubiquitin chains linked through lysine 63. Results provide support for a model in which an Mms/Ubc 13p complex assembles novel polyubiquitin chains for signaling in DNA repair.

DNA repair, Saccharomyces, Ubiquitin

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Structure of a HoxB1-Pbx1 heterodimer bound to DNA: role of the hexapeptide and a fourth homeodomain helix in complex formation

Article Abstract:

Hox homeodomain proteins are developmental regulators that determine body plan in several organisms. Most of vertebrate Hox proteins bind DNA as heterodimers with the Pbx1 homeodomain protein. The 2.35 angstrom structure of a ternary complex with a human HoxB1-Pbx1 heterodimer bound to DNA was investigated. Heterodimer contacts are mediated by the hexapeptide of HoxB1, which binds in a pocket in the Pbx1 protein formed in part by a three-amino acid insertion in the Pbx1 homeodomain.

DNA, Proteins

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