Nuclear mutations in Saccharomyces cerevisiae that affect the escape of DNA from mitochondria to the nucleus

Article Abstract:

The escape process by which DNA travels from the mitochondria to the nucleus was examined by inserting a nuclear DNA fragment bearing TRP1 and ARS1 into a functional yeast mitochondrial chromosome in which trp1 has been deleted. The TRP(super -) strain produced Trp(super +) clones having the TRP1/ARS1 fragment, proof that DNA migrated from the mitochondria to the nucleus. 21 recessive mutants whose DNA escape rates rise with screening for increased papillation rates to Trp(super +) were isolated and classified into six complementation groups, designated as YME1-YME6. Analysis revealed that yme1, yme2, yme4 and yme6 mutations increase mitochondrial escape by altering mitochondrial functions.

Mitochondria, Mitochondrial DNA, Mutation (Biology), Mutation

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Direct cloning of yeast genes from an ordered set of lambda clones in Saccharomyces cerevisiae by recombination in vivo

Article Abstract:

A technique for cloning yeast genes which uses a plasma vector for rescuing lambda clones as yeast centromere plasmids is presented. The system solves cloning problems caused by yeast genes' underrepresentation in genomic libraries, highly marginal phenotypes that prevent selection through genetic complementation and phenotypes requiring laborious scoring. The utility of the technique is demonstrated by using it to clone the GAL83, SSN2 and MAK7 genes.

Methods, Usage, Cloning, Genetic vectors

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