Polyunsaturated fatty acid biosynthesis in Saccharomyces cerevisiae: expression of ethanol tolerance and the FAD2 gene from Arabidopsis thaliana

Article Abstract:

The introduction of an inducible Arabidopsis thaliana fatty acid desaturase gene, FAD2, into Saccharomyces cerevisiae, causes the endogenous synthesis of polyunsaturated fatty acids. The FAD2 protein uses oleoyl (18:1) and palmitoleoyl (16:1) residue binding phosphatidylcholine (PC) as substrates, converting them to 18:2-PC and 16:2-PC respectively. FAD2 transformants grown at 8 degree centigrade contain more of unsaturated fatty acyl residues and less of polyunsaturated fatty acyl residues. Cells expressing FAD2 are more resist to ethanol.

Physiological aspects, Observations, Microbiological synthesis, Gene expression, Unsaturated fatty acids, Fatty acid desaturases

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Screening of genes involved in isooctane tolerance in Saccharomyces cerevisiae by using mRNA differential display

Article Abstract:

Several genes are involved in the isooctane tolerance of Saccharomyces cerevisiae strain KK-211. Some of the genes respond to cell stress, some are involved in the uptake of trehalose and the production of glycogen, and some are involved with cell surface maintenance.

Organic solvents

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Induction of a mitosis delay and cell lysis by high-level secretion of mouse alpha-amylase from Saccharomyces cerevisiae

Article Abstract:

Excessive production of alpha-amylase in Saccharomyces cerevisiae delays mitosis and causes cell lysis. It does this by downregulating the production of protein phosphatase 2A, maturation-promoting factor, and PPH22.

Cell cycle, Amylases

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