One group of genetically similar Listeria monocytogenes strains frequently dominates and persists in several fish slaughter- and smoke-houses

Article Abstract:

A study was undertaken to determine whether genetically similar strains colonize different processing plants or whether specific persistent strains are unique to each processing plant and samples were collected from four fish smokehouses and four fish slaughter houses. The study indicates that strains of Listeria monocytogenes that are genetically very closely related may be especially adapted to colonizing the processing equipment or especially resistant to cleaning and disinfection.

Science & research, Environmental aspects, Genetic aspects, Food contamination, Listeria monocytogenes, Slaughtering and slaughter-houses, Slaughterhouses

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Differentiation of Shewanella putrefaciens and Shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16S rRNA gene sequence analysis

Article Abstract:

Seventy-six Shewanella putrefaciens isolates from fish, oil drillings and clinical specimens, the type strain of S. putrefaciens (ATCC 8071), the type strain of S. alga (IAM 14159), and the type strain of S. hanedai (ATCC 33224) were contrasted by various typing techniques. Findings showed that the isolates must be considered two different species, S. alga and S. putrefaciens, and that most mesophilic isolates previously identified as S. putrefaciens belong to S. alga.

Gram-negative bacteria

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Differentiation of Campylobacter coli and C. jejuni by length and DNA sequence of the 16S-23S rRNA internal spacer region

Article Abstract:

A study was conducted to analyze the internal spacer region between the 16S and 23S rRNA genes of Campylobacter. Polymerase chain reaction fragment length typing and DNA sequencing were utilized to carry out the analysis. Experimental results indicated diverging DNA sequences between all the strains. Findings also showed deletions within regions of the reference strains NCTC 11353 and NCTC 11392 that may be correlated with genomic rearrangement.

Usage, DNA, Campylobacter, Polymerase chain reaction, RNA

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