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Oxidation of benzene to phenol, catechol, and 1,2,3-trihydroxybenzene by toluene 4-monooxygenase of Pseudomonas mendocina KR1 and toluene 3-monooxygenase of Ralstonia pickettii PKO1

Article Abstract:

The consecutive hydroxylations led to the invention of toluene 4-monooxygenase (T4MO) of Pseudomonas mendocina KR1, toluene 3-monooxygenase (T3MO) of Ralstonia pickettii PKO1 and toluene ortho-monooxygenase(TOM) of Burkholderia cepacia G4 that changed benzene to phenol, catechol and 1,2,3-trihydroxybenzene. The very heavy liquid chromatography and mass spectrometry together were used to discover the catechol and 1,2,3-trihydroxybenzene products.

Author: Bentley, William E., Wood, Thomas K., Tao, Ying, Fishman, Ayelet
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Petrochemical Manufacturing, Benzene, Analysis, Usage, Oxidation-reduction reaction, Oxidation-reduction reactions, Chromatography, Properties

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Modification of spatial distribution of 2,4-dichlorophenoxyacetic acid degrader microhabitats during growth in soil columns

Article Abstract:

The spatial distribution of 2,4-dichlorophenoxyacetic acid (2,4-D) degrader microhabitats as a function of 2,4-D degrader abundance is investigated by using a soil microsampling strategy combined with a mathematical spatial analysis. The findings indicate that the characterization of bacterial microscale spatial distribution is relevant to microbial ecology studies.

Author: Pallud, C., Dechesne, A., Gaudet, J. P., Debouzie, D., Grundmann, G. L.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Acetic Acid, Bacteria

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Subjects list: Research, Microbiology
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