Degradation of nitrobenzene by a Pseudomonas pseudoalcaligenes

Article Abstract:

A metabolic pathway causes the breakdown of nitrobenzene to nitrosobenzene, and later to hydroxylaminobenzene. Each step requires grown cells, under aerobic and anaerobic conditions, and transforms nitorbenzene to 2-aminophenol with the oxidation of 2 mol of NADPH. Ring cleavage, requiring ferrous iron, gave a transient yellow product with a maximum A-380. In the presence of NAD, the product vanished and NADH was obtained. The absence of NAD led to the spontaneous conversion of the ring fission product to picolinic acid.

Analysis, Nitrobenzenes

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Oxidation of nitrotoluenes by toluene dioxygenase: evidence for a monooxygenase reaction

Article Abstract:

Toluene-grown cells of Pseudomonas putida F1 and Pseudomonas sp. JS150 oxidized 2- and 3-nitrotoluene to 2- and 3-nitrobenzyl alcohol. These results indicate that toluene dioxygenase was responsible for oxidizing the methyl substituent. Incubation of these cells with 4-nitrotoluene resulted in production of 2-methyl-5-nitrophenol and 3-methyl-6-nitrocatechol. A possible mechanism for this reaction was elucidated, with oxidation by toluene dioxygenase as the initial step.

Enzymes, Pseudomonas putida

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Initial reductive reactions in aerobic microbial metabolism of 2,4,6-trinitrotoluene

Article Abstract:

Research was performed on Pseudomonas sp. clone and trinitrotoluene (TNT)-8 and TNT-32 to determine the significance of the 2,4,6-trinitrotoluene pathway. Evidence suggests that initial microbial transformations of 2,4,6-TNT are characterized largely by reductive rather than oxidation reactions due to high electron deficiency. Furthermore, formation of hydride complexes could not be identified with the TNT-enriched strains of TNT-8 and TNT-32 or with Pseudomonas clone A.

Bacteria, Nitrifying, Nitrobacteraceae

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