Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification

Article Abstract:

Amplification of 16S rRNA from mycoplasmal organisms using the polymerase chain reaction allowed the identification of species- and genus-specific sequences suitable for identifying the organisms. The species-specific sequences hybridized only with the corresponding species. The genus-specific sequence was highly specific for mycoplasma but also cross-reacted with some related organisms. The V2, V3, V5 and V7 regions of the 16S rRNA were used to develop the species-specific primers, while the genus-specific primer was obtained from nucleotides 1029 to 1055.

Nucleotide sequence, Base sequence

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Application of a Mycoplasma group-specific PCR for monitoring decontamination of Mycoplasma-infected Chlamydia sp. strains

Article Abstract:

Incubation with Triton X-100 is useful in the decontamination of the laboratory strains of Chlamydia pneumoniae, C. pecorum and C. trachomatis, infected with Mycoplasma. A Mycoplasma group-specific PCR technique detects the contamination of cell lines. The contamination with Mycoplasma spp. changes the experiment parameters of Chlamydia strains and necessitates a Mycoplasma-free material. Antibodies cannot be used against mycoplasma as they suppress the Chlamydia cells.

Observations, Cell lines, Microbial contamination, Chlamydia

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Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction

Article Abstract:

The detection of Campylobacter spp. in chicken products usinga combination of short enrichment culturee and polymerase chain reaction (PCR) is discussed. This involved the development of a specific PCR and probe hybridization assay based on the 16S rRNA gene sequence. A comparison of the method with conventional detection techniques yielded the same results. However, the results from the PCR-culture assay was obtained faster.

Campylobacter

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